Advantages Of jis z 2801 iso 22196 Test for Various Product 



When we talk about competence and importance while creating the offer, we refer to the technical binomial of development versus competent laboratory within the framework of product development. This implies several premises of a specialized and social nature: knowledge of the standards involved in product development, knowledge of test tools and machines, the conditions for product certification and its environment, the hypothetical deployment in other countries with climatic conditions different from those of the country of origin of the certificate, as well as other local requirements that must be taken into account in the process and national deviations in the applicable regulations.

jis z 2801 iso 22196 is an antibacterial surface method that tests antibacterial activity and antibacterial effect. Japanese Industrial Standards (JIS) Z 2801 is a commonly required quantitative antibacterial test for plastics, foams, and fibers and is in harmony with ISO 22196. The standard method tests hard surfaces and plastics to measure the growth that may occur after that. The standard test is 24 hours. It is one of the most demanding, fast, and reliable antibacterial surface tests to determine antibacterial activity and antibacterial effect.

The JIS Z2801 and international standard ISO 22196 are recognized approaches for assessing the effectiveness of antibacterial products when applied to surface materials such as plastics, ceramics, and materials.

The Durability testing and this test can provide essential information about the antibacterial growth and performance.

Advantages of the JIS Z 2801 test method:

  • The JIS standard is globally recognized and is a highly reliable antibacterial test.
  • JIS standards are standardized in a wide range of markets.
  • The harmonized version of JIS Z 2801, ISO 22196, is almost the same as JIS Z 2801 test method.

At the Testing Lab, we focus on high-quality testing and believe that understanding project requirements and complete communication is essential for every project. Whether you choose the correct test method or establish a quality control program, experienced technical and commercial staff can assist and initiate product testing that requires it. The laboratory can be used for rapid and reliable antibacterial testing to support product development, performance testing, and ongoing quality control.

The test can be performed on Staphylococcus aureus, ATCC 6538P, E. coli ATCC 8739 strains, and other strains upon request. In 2007, the JIS Z2801 method was internationally standardized as ISO 22196.

JIS Z 2801 sample requirements:

  • The sample size should be 5 x 5 cm.
  • Each measurement setup requires at least six blank samples.
  • A single measurement requires a sample with at least three antibiotics.

Paints / colors / lacquers / coatings can be delivered in liquid form. Paint the LENETA test plate (as a support) for paint / color / lacquer / coating.

Pack the sample and label the sample lot, serial number, or information you want to convey about the content accordingly.

Do not mark the sample with a felt-tip pen or marker.

Those colors are easily blurred and often result in improper colors. This also applies to oil-based markers. If you need to label the sample, use a pencil.

The procedure of the JIS Z 2801 test method:

-Test organisms are extracted directly from the agar plate and diluted with buffer

-Microbial concentrations are standardized by diluting with nutrient broth

-Inoculate the control and test surfaces with 0.4 ml of bacterial suspension three times.

-The inoculums are fully covered with a protective film to prevent evaporation of the suspension.

-Immediately after inoculation, the bacteria in the reference sample are isolated from the sample surface. The number of viable bacteria (t0 value) is determined by elution followed by dilution and plating.

-Other samples (t24) are incubated in a humid environment for 24 hours.

-Activity/efficacy is calculated by comparing the concentration of the blank after 24 hours with the concentration of bacteria after 24 hours

Strengths of the JIS Z 2801 test method:

-Sufficient reproducibility and reproducibility (ISO shows at least 60% variation)

Weaknesses of the JIS Z 2801 test method:

-Not very suitable for hydrophobic samples

-According to the standard, the sample should be very large: 5 x 5 cm

-The surface of the sample should be smooth and smooth

-Curved samples are only partially suitable

-Quite medium / low sensitivity

The Japanese Industrial Standards Committee (JIS) is an international organization that develops and standardizes test methods for various products and materials. This method can be performed using a contact time in the range of 10 minutes to 24 hours. In the JIS Z 2801 test, the non-antibacterial control surface is used as the basis for microbial reduction calculations. JIS Z 2801 Specific laboratory qualification

The institute started implementing the JIS Z 2801 test method in 2007. Since then, the laboratory has conducted thousands of JIS Z 2801 tests on a wide range of test materials against countless bacteria, fungi, and viruses. The lab is trained in changing methods to meet the needs of our clients. Each JISZ 2801 laboratory will be conducted in a manner suitable for the test substance submitted by the sponsor while maintaining the integrity of the study.

The competence of a laboratory from the point of view of the offer creation process is based on two clearly defined concepts: the number of accreditations related to their age and the experience of the technical staff and their knowledge of the technologies to be developed within the laboratory.

Outline of procedure

  • Test organisms are usually prepared by growth in a liquid medium.
  • Test microbial suspensions are standardized by diluting with nutrient broth (this is

It allows microorganisms to grow during the test).

  • Inoculate the surface of the control and test with microorganisms and then inoculate with microorganisms.

The inoculums is covered with a thin, sterile film. Covering the inoculums spreads it and prevents it.

Evaporates and ensures close contact with antibacterial surfaces.

  • Microbial concentration is determined by elution followed by dilution at “zero time.”

Agar plating.

  • Inoculated and covered antimicrobials and control test surfaces can be incubated without touch

Twenty-four hours in a humid environment, usually at body temperature.

  • After incubation, microbial concentration is measured. Reduction of microorganisms

Calculated relative to the control surface.

How accurate are the results?

Some argue that this method has some limitations and is not an accurate representation of the contamination event. The treated surface may meet the requirements of ISO 22196, but “real-life” studies may not show the same results.

It is worth considering that ISO 22196 can be run under “real” laboratory conditions. However, this method provides the bacteria with ideal growth conditions, otherwise interfering with the material’s performance and allowing control of diseases that could obscure it. In “real life,” you cannot control or know the number of bacteria that have grown and what they have been reduced to. Also, it is not possible to tell if the control was treated the same as the test sample. As a result, it provides a viable alternative for assessing whether a product offers bacterial reduction.

Various environmental conditions can be changed according to customer needs, such as inoculums concentration, UV irradiation, and shortened contact time. This allows you to simulate different situations, but the controlled lab test environment ensures reproducible results.